By Dr. Jan M. Anderson (auth.), C. Sybesma (eds.)
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Extra resources for Advances in Photosynthesis Research: Proceedings of the VIth International Congress on Photosynthesis, Brussels, Belgium, August 1–6, 1983
J <( 450 550 A(nml 750 390 450 550 650 750 A(nml Figure 3. Linear dichroism ( - - or ---) and absorption ( ... ) spectra of the cytochrome b 6 f complex oriented in squeezed polyacrylamide gel at room temperature. - 3a Top : untreated sample Middle and bottom : sample after dithionite treatment. The absorption and negative linear dichroism band around 670 nm are caused by a contamination of the cytochrome preparation by chlorophyll in detergent micelles. - 3b Dithionite treated-minus-untreated difference spectra revealing the absorption and linear dichroism of cytochrome b6 .
93 3 Randomized normal ves. 10 4 Stromal lamellae t1;2,ms Mg effect MV effect 21 By comparison with thylakoids, one of the feature of Fig. 2 is the very high fluorescence in granal inverted thylakoids and the very low in stromal lamellae. This stresses their respective SII and SI origin and the almost entirely SII character of the fluorescence at this wavelength, including most F0 . Another point is the minute effect of Mgcl 2 on the granal inverted vesicles, compared to the two-fold F increase in thylakoids.
Et al. 1982 ; Haworth P. et al. 1982b) that the state of chl b in LHC is identical to its in vivo state. , unpublished). Accordingly it is tempting to speculate that the change in LD observed (fig. 2b) upon treatment of LHC by SDS reflects a modification of the orientation of a chl b molecule (or a perturbation of the interactions between the cluster of chl b in the trimer) brought about by the change in the bonding pattern of the chl b molecules. 1. , Tapie P. 1983) and parallel to the membrane plane in the case of LHC reconstituted in lipid vesicles (Nabedryk E.